You can disable this option from the TestStand Sequence Editor by completing the following steps.
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From within the TestStand Sequence Editor, select Debug >> Sequence Analyzer >> Sequence Analyzer Options.
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When prompted with the following dialog box, disable the option Analyze File Before Executing and select OK to confirm.

Disabling this option can be useful to avoid initial errors, for example if you are creating variables during the execution of your sequence file.