Next Steps
Note that the above two methods are customizable and are not the only solutions that can be used. The methods are applicable when the user run the sequence.
If you need to automatically show the error or prevent the user from executing the sequence file, you may use the engine callback to do so. To use the engine callback, you can add the SequenceFileLoad engine callback at the sequence pane. Then add the methods above into this SequenceFileLoad engine callback. Whenever the sequence file has expired or reached the set timeline, once opened, it will immediately abort or throw an error to disable the user from executing the sequence file.
Also, if you need to set a password-protected for the sequence file, you may refer to the article: Lock TestStand Sequence File from Editing or Viewing.
If you are using the LabVIEW adapter, consider to lock the block diagram of the LabVIEW as mentioned in this article: Locking The Block Diagram in LabVIEW instead of locking the whole sequence file. You could change the timestamp control to a constant so that the user is not able to change the set timeline in the block diagram.