In Windows, the steps to perform are listed as shown:
- Navigate to the following folder path: C:\Program Files (x86)\National Instruments\LabVIEW 2025. For MacOS you may refer to the following link below: Open the LabVIEW configuration file (LabVIEW.ini)
- Open the configuration file as shown in the circled red box as shown below.
- Add the following ini key to the configuration file.
Editor.Zoom.BlockDiagram.Enabled=False
- Save the configuration file and restart LabVIEW. The changes will be applied.

Do take note that by inserting this into the LabVIEW configuration file, not only it will disable the zooming feature via keyboard shortcut, but it will also disable the zoom feature via the View menu bar in LabVIEW.
To maintain the zooming feature:
- If you want to maintain the zooming feature via the View menu, View>>Zoom In/Zoom Out, but disable the <Ctrl-Mouse Wheel> keyboard shortcut for zooming block diagram, you can add Editor.Zoom.MouseWheelGestureRequireShift=True to the configuration file as shown below.
- Line of code to add in to the configuration file
Editor.Zoom.MouseWheelGestureRequireShift=True
- Save the configuration file and restart LabVIEW. The changes will be applied.
- By adding this, the new keyboard shortcut for zooming will be changed to <Ctrl-Shift-Mouse Wheel>, while the original keyboard shortcut <Ctrl-Mouse Wheel> feature will be changed to changing sub diagrams for case structures, event structures etc.