Solution
The .CR2 images are not supported natively by NI's Vision software (VAS or VDM).
To read them in using IMAQ ReadFile.vi, for example, you would first need to convert them to a supported image format (PNG, JPEG, BMP, or TIFF) using a 3rd-party conversion tool.
Here's more information about
NI Vision File Formats and Image Type Compatibility .