You can use the property of the Vision Assistant called the ROI Descriptor. To access this property in Vision Assistant, you will make a step that uses the ROI. Then click Set Controls.
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When you are in the controls section, make sure ROI Descriptor is selected.
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After that you will be able to change the values of the ROI. You can also create a ROI property node from your Image Indicator to read a drawn ROI from the front panel. You should do this for the image you acquire before processing with the Vision Assistant. You will need to create a second Image Indicator after the Vision Assistant to see the results of your ROI changes.
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