Yes, a VI that is written with LabVIEW 64-bit development system can be opened in LabVIEW 32-bit and vice versa because the code is not compiled until the VI is run or saved.
The VI source code is portable, but execution depends on the availability of compatible dependencies.Executables (EXEs) are not interchangeable between 32-bit and 64-bit systems without rebuilding for the target architecture.
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