Additional Information
With X Series devices, only Port 0 can be used with a sample clock, enabling hardware-timed digital I/O. Other ports lack access to this timing circuitry and therefore cannot be used for hardware-timed tasks. Attempting to use any port other than Port 0 for such tasks will trigger the error.
The distinction between Port 0 lines and PFI lines will be outlined in the specifications document for your NI Device. An example of the PXIe-6375 is shown below.



You can read more on the difference between Digital I/O and PFI in chapters 6 and 8 of the X Series User Manual , respectively.