You can use
Call Library Function Node and make use of user32.dll functions.
- Drop Call Library Function Node to the block diagram, then right click>>Configure...
- Set user32.dll on Library name or path.
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- For Function name, you can use one of below
- On Parameters tab, add parameters corresponds to the function that you chose based on the Syntax which you can find on the links above or Microsoft page.
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Below is a sample VI which showcase the use of
Call Library Function Node set mouse cursor and then performs mouse click action.