Additional Information
The I/O Server makes calls to the OPC server through DCOM, even if not using a remote OPC server. The DCOM user checking can block all user names that are not registered with the server. When using DSC as an OPC client, the NI Shared Variable Engine (SVE) is the application that makes the connection to the OPC server. The SVE is a system service and, by default, logs and runs as the local system user.
The communication can be rejected if the user name of the service is system. As long as the I/O Server is still deployed, it will continue to try to connect with the OPC server and fail, and shared variables will return the Server Failure error or no data at all. To avoid this issue, change either of the following settings.
Prior to LabVIEW 2012, NI Variable Engine is listed as National Instruments Variable Engine in Windows Services. This behavior is known to occur with Campbell Scientific OPC Servers and Takebishi's DeviceXPlorer OPC Server.
If none of the above steps work, try using another OPC Server to rule out that the problem is related to this one.