Additional Information
A breakpoint is one of the tools that you can use to debug your LabVIEW VI. Normally, you would use this tool on a wire to pause the execution of your VI at a particular point, but it is very easy to accidentally click the block diagram itself, instead of a wire.
If this happens, a red outline will appear around your block diagram, and this will effectively pause your VI before anything on your block diagram executes. This is helpful, if you want to step through your VI while debugging, but it can make your VI behave strangely when you try to run it normally.